As shown in Figures 3E, F, DPT significantly decreased HIF-1α-dependent luciferase activity following hypoxia treatment in both cell lines.Furthermore, we examined the expression ofCA IX, which is a specific target gene of HIF-1α and is a key mediator of tumor progression (30).As shown in Figures 3G, H, DPT treatment decreased CA IX mRNA expression compared with the control treatment. This evidence concerns the gene CA9 and neoplasm.