Lastly, authors investigated T cells subsets and gene expression in double immune checkpoint expressing cells, confirming expression of genes inhibiting T-cell activation in CD4+ memory T cells co-expressing TNFRSF18 and TNFRSF4. Overall, droplet-based scRNA-Seq with 10,000 cells only demonstrated its power to analyze tumor heterogeneity and infiltrating T cells phenotype. Here, CD4 is linked to neoplasm.