In 2004 a pooled analysis of the data from NINDS, ECASS, and ATLANTIS trials suggested two interesting conclusions: [1] a greater benefit was observed when tPA administered to AIS patients within 90 min of stroke onset, upholding the concept of “sooner the better” and [2] a potential benefit beyond 3 h of tPA administration was also observed, however with some risks (23). This evidence concerns the gene PLAT and stroke disorder.