As shown in Figure 6, the mRNA relative expressions of NF-κB, TNF-α, and IL-1β of HF zebrafish in the model control group were significantly increased compared with those of HF zebrafish in the normal control group (16.65 ± 0.95 versus 1.00 ± 0.08, p < 0.001; 1,204.50 ± 46.78 versus 1.00 ± 0.30, p < 0.001; 70,859.65 ± 6,903.64 versus 1.00 ± 0.08, p < 0.001). The gene discussed is TNF; the disease is hydrops fetalis.