Results of our previous study demonstrated that peripheral abundance of nesfatin-1 could be changed by high-fat diet and chronic stress, and ip injection of nesfatin-1 could induce anxiety and depression-like behavioral disorder in rats, together with the change of hippocampal synaptic plasticity (Ge et al., 2015; Chen et al., 2019). Here, NUCB2 is linked to depressive symptom measurement.