To determine the long-term impact heterozygous SKP1 loss has on CIN, nuclear area, MN formation and chromosome numbers were assessed in SKP1+/− 1, SKP1+/− 2 and NT-Control clones at regular time intervals (i.e., every four passages) for ~3 months. This evidence concerns the gene SKP1 and cervical squamous intraepithelial neoplasia.