To confirm the role of Rab26 in regulating migration/invasion of breast cancer cells, we tried to deplete Rab26 through lentivirus-mediated shRNA expression system in lower invasive and Rab26 higher-expressed MCF7 cells (Fig. 3A, shRNA-Rab26-1 was used in the following experiments as it efficiently depletes Rab26). The gene discussed is RAB26; the disease is breast cancer.