KLF5 and posterior cortical atrophy: Consistent with findings from the co-culture system, CM from cells expressing KLF5KR significantly reduced mature osteoclasts, as indicated by fewer TRAP + cells (Fig. 4f, g) and reduced expression of osteoclast markers (Fig. 4h), further indicating that interruption of KLF5 acetylation in PCa cells suppresses osteoclast differentiation.