However, CM from DU 145 PCa cells with different forms of KLF5 did not affect osteoblast differentiation, either in normal or differentiation medium, as indicated by Alizarin red S staining (Supplementary Fig. 6h–j) and expression of osteoblast differentiation markers (Supplementary Fig. 6k). This evidence concerns the gene KLF5 and posterior cortical atrophy.