Consistent with the induction of EMT by the knockdown of KLF5 in immortalized epithelial cells46, deletion of KLF5 in PC-3 PCa cells induced EMT even without TGF-β, as indicated by the mesenchymal morphology, downregulation of epithelial marker E-cadherin, upregulation of mesenchymal markers N-cadherin and vimentin, and enhanced cell migration and invasion (Supplementary Fig. 3h–j). This evidence concerns the gene VIM and posterior cortical atrophy.