SHH and neoplasm: In this study, although knockdown of SHH in KLF5KQ cells did not show an obvious effect on the differentiation of RAW264.7 preosteoclasts (Fig. 7d), co-cultures of KLF5- and KLF5KQ-expressing tumor cells with MC3T3-E1 preosteoblasts increased IL-6 expression in MC3T3-E1 cells, and CM from the co-cultures enhanced the differentiation of RAW264.7 preosteoclasts (Supplementary Figs. 6k and 8h, i).