To further clarify the source of upregulated PKM2 in CAFs induced by gastric cancer exosomes, we first constructed a PKM2-eGFP fusion protein gene, transfected it into AGS cells, screened for the AGS cell line stably expressing PKM2-eGFP, extracted exosomes, and then added these exosomes to the culture system of CAFs differentiated from MSCs by gastric cancer exosomes. Here, PKM is linked to gastric cancer.