In addition, we overexpressed C20orf204-189AA in Huh7 cells, a differentiation grade G1 HCC cell line that expresses C20orf204 at low level5, and these cells also showed an increase in proliferation (1.5-fold; p = 0.002) (Fig. S2). The gene discussed is C20orf204; the disease is hepatocellular carcinoma.