The ELISA evaluation detected only 33.34% of the antibodies (5.56% IgG+/IgM+, 11.11% IgG−/IgM+, and 16.67% IgG+/IgM-) while the nested PCR assay detected 80.56% that were specific to the B1 toxoplasmosis gene (Table 2). Here, CD40LG is linked to toxoplasmosis.