The first round of PCR produced amplicons with a size of 198 bp for all samples of IgG+/IgM+, IgG−/IgM+, IgG+/IgM-, and 51 samples (47.2%) of IgG−/IgM- (24Mo, 18 N and 9A); therefore the PCR detected toxoplasmosis antibodies in a total of 87 (80.56%) of the 108 samples (Table 2, Fig. 1), 12 of these samples, represented 100% of female A participants, though 21 (19.4%) samples (3Mo, 18 N and 6A) showed negative PCR products and IgG−/IgM-. Here, CD40LG is linked to toxoplasmosis.