In 2003, Zhao et al. proposed that IPO is also an effective method to protect the ischemic myocardium from MIRI [26], the mechanism, which IPO exerts protection may be due to the fact that IPO itself can delay sympathetic nerve activation during ischemia and reduce the opening of mitochondrial KATP-sensitive potassium channels [27], as well as increase the phosphorylation level of endothelial nitric oxide synthase to protect the myocardium from damage [28]. The gene discussed is NOS3; the disease is ischemia.