To further investigate the roles of WDR81 in selective autophagy in HD, we examined the recruitment of endogenous WDR81 in HeLa cells stably expressing Htt97Q-EGFP, a mutant Huntingtin protein (Htt) which harbors an expanded polyglutamine (polyQ) repeat and forms Htt polyQ aggregates [12, 24–27]. Here, WDR81 is linked to Huntington disease.