Hence, we investigated the persistence, NO inhibition, iNOS expression, TGF-β signaling, and LC3 loading to the PV, using four strains of T. gondii of different genetic backgrounds (RH, ME-49, VEG, P-Br) after infection of activated mice peritoneal macrophages to determine if NO inhibition is a possible general immune evasion strategy for this parasite. This evidence concerns the gene MAP1LC3A and infection.