Moreover, to further confirm the effect of DHX32/β-catenin pathway on HCC proliferation and EMT in vivo, we should also detect the expression of proliferation makers, such as PCNA, Cyclin D1, β-catenin expression, and EMT markers including E-cadherin and vimentin in the tumour tissues. This evidence concerns the gene CCND1 and hepatocellular carcinoma.