TNF and Sepsis: After 2 weeks of sepsis induction in the presence of ovalbumin (OVA) as an antigen, cytokine production of splenocytes was analyzed in vitro, showing that the J-1012 N-glycan enhanced antigen-specific IFN-γ production, which possibly supported a Th1 reaction in DTH25; notably, however, IL-10, TNF-α, and IL-6 production were not enhanced (Fig. 4b).