GCG and Hypoglycemia: Plasma glucagon increased approximately six-fold for CON (89.8 pM (67.7–118.5)) and three-fold for DIA (46.3 pM (20.5–70.9)) compared to baseline (CON: 14.9 pM (9.6–25.3), DIA: 14.8 (14.4–21.2)) in both groups during the CLAMP, with highest levels after 30 min (t210) of hypoglycaemia, with significantly higher levels in CON compared to DIA (see Fig. 2f).