As expected, infection of NRVMs with recombinant adenovirus driving expression of a constitutively active FoxO1 mutant (AdcaFoxO1) resulted in a robust increase in FoxO1 transcript and its gene-dependent transcriptional program, whereas control infection with AdGFP had no effect (Supplementary Fig. 4e, f). This evidence concerns the gene FOXO1 and infection.