Further, we downregulated and overregulated the expression of EIF5A2 in ovarian cancer cells and found that the expression of the related stem genes, spheroid formation ability, chemotherapeutic drug sensitivity, and in vivo different gradients tumorigenicity were attenuated or strengthened, suggesting that EIF5A2 functions in maintaining the stemness of ovarian cancer cells, which is demonstrated for the first time. This evidence concerns the gene EIF5A2 and ovarian cancer.