Our function rescue experiments showed that some deprived CSC properties induced by EIF5A2 knockdown, such as decreased expression of stem markers, inhibited spheroid forming ability, and reduced chemo-resistance and tumorigenicity, were partially reversed by KLF4 overexpression, suggesting that KLF4 participates in the process of EIF5A2 modulating stemness in ovarian cancer cells. Here, KLF4 is linked to ovarian cancer.