In the allele model, we found that rs2277698 (TIMP2) significantly reduced the risk of osteosarcoma in males (OR = 0.57, 95% confidence interval [95% CI] = 0.25–0.9, P = .006; OR = 0.35, 95% CI = 0.26–0.77, P = .029, females (OR = 0.52, 95% CI = 0.33–0.85, P = .041), people aged under 24 (OR = 0.43, 95% CI = 0.26–0.91, P = .037; OR = 32, 95%CI = 0.21–0.68, P = .028), and the population over 56 years of age (OR = 0.51, 95% CI = 0.24–0.76, P = .018; OR = 0.43, 95% CI = 0.23–0.81, P = .047). The gene discussed is TIMP2; the disease is osteosarcoma.