PTEN and adenocarcinoma: Histologically, LvCaP-2 and LvCaP-2R are high-grade adenocarcinomas, which genetically have a hemizygous loss-of-function (LOF) truncating mutation in TP53 (T211fs) and hemizygous deleterious mutation (R130Q) in PTEN with a loss of PTEN protein expression (29).