The participation of NEO1 in NB cell migration, together with the fact that NEO1 knock-down cells were less metastatic in a chorioallantoic membrane assay [10], led us to evaluate in this work the potential role of NEO1 in NB metastasis using a immunodeficient mouse model and a spontaneous metastasis approach. The gene discussed is NEO1; the disease is neuroblastoma.