DNMT1 suppression by pretreatment with DNMT1 siRNA or the administration of RG108, a selective DNMT1 inhibitor, significantly protected against noise-associated OHC and IHC synaptic ribbon losses and attenuated NIHL in adult mice, suggesting epigenetic modifications are responsible for hearing loss and may provide a good candidate for future therapy aimed at NIHL. This evidence concerns the gene DNMT1 and noise induced hearing loss.