Mitsubishi-Tanabe subsequently published data regarding more potent thienodiazepine derivatives in 2016, including compound ‘7f’, which potently inhibits tumour growth at 20 mg/kg.40 Differential scanning fluorimetry revealed that, of the 41 human bromodomain-containing proteins that exist, JQ1 binds with the highest affinity to BRD4.23 Crystallographical studies demonstrate that this small molecule mimics acetylated lysine and competitively fits into the binding pocket of the BET bromodomains,23 forming a hydrogen bond with a conserved asparagine, and shows affinity for both BD1 and BD2. This evidence concerns the gene DNER and neoplasm.