In the last series of experiments, the impact of removing S6K1 was examined on depression-related behavior using readouts for lack of motivation (nest building, Fig. 5a, grooming in the splash test41, Fig. 5b, c), anhedonia (sucrose preference, Fig. 5d), or resignation (forced swim test, Fig. 5e, f). The gene discussed is RPS6KB1; the disease is major depressive disorder.