To study the contribution of decreased INSIG1 in the early stages of NASH, we exposed Insig1 KO mice and their wild-type (WT) and heterozygous (Het) littermates to a Western diet (high in saturated fat, cholesterol, and refined carbohydrates) supplemented with sugar water (WDSW) ad libitum, thus promoting activation of SREBP1 and ChREBP transcriptional programmes [30]. This evidence concerns the gene SREBF1 and metabolic dysfunction-associated steatohepatitis.