Mice deficient in both Il4 and Il13 (Il4-/-Il13-/-) were injected with a liver-specific AAV expressing Pcsk9 to result in LDLr deficiency and fed Western diet (WD) to promote hypercholesterolemia in order to develop advanced atherosclerotic lesions by 20 weeks (Bjørklund et al., 2014; Roche-Molina et al., 2015; Peled et al., 2017). The gene discussed is PCSK9; the disease is Hypercholesterolemia.