To clarify whether PP4 has conserved functions in meiosis in mammalian species, we generated its catalytic subunit gene Ppp4c conditional knockout (Ppp4cf/f) mouse strain using CRISPR/Cas9 technology, and showed that loss of PPP4C did not affect male germ cell meiosis, acrosome formation, nuclear condensation and elongation, but caused the defect of cytoplasm removal, which in turn leads to the failure of spermiogenesis completion and male infertility (Han et al., 2020). This evidence concerns the gene PPP4C and male infertility.