In this study, we found that the expression of the ER stress related proteins PERK, Calnexin, Ero1-l α, BIP, and PDI was significantly higher in the Ad-Vp3-infected HepG-2 cells compared to Ad-Mock-infected HepG-2 cells, 12 h and 24 h post-infection. This evidence concerns the gene ERO1A and infection.