The expression of lipid metabolism related enzymes ACC, FASN, PLD1, p-PLD1, and SCD1 in Ad-Vp3-infected cells were significantly higher at 12 and 24 h, and significantly lower at 36, 48, and 72 h post-infection when compared with the Ad-Mock infected cells (p < 0.05). This evidence concerns the gene PLD1 and infection.