To identify genes that were epigenetically silenced by STAT3 activation, we performed a sequencing based method (MBDCap-Seq) in AGS gastric cancer cell line (an adenocarcinoma with constitutive STAT3 activation), depleted of STAT3 by viral-based shRNA (9), to compare methylation changes in this cell and the control cells (Figure 1A). The gene discussed is STAT3; the disease is gastric cancer.