To further confirm whether Santacruzamate A and MIR22HG affect the radiosensitivity of liver cancer in vivo, we established the NC group and radiation 4 Gy only treated group, radiation 4 Gy + Santacruzamate A group, and radiation 4 Gy + Santacruzamate A + MIR22HG knockout group. The gene discussed is MIR22HG; the disease is liver cancer.