To explore whether EZH2 regulates Et-miRNAs through histone H3K27 trimethylation on the Et-miRNA promoter, we carried out the CHIP assay near the transcription start site (TSS) on the Et-miRNA promoter region using the EZH2 and H3K27me3 antibodies in the ovarian cancer cell line A2780. This evidence concerns the gene EZH2 and ovarian cancer.