Subsequently, to delineate the mechanism by which this process takes place, we performed focused PCR array profiling on 84 targeted genes related to the extracellular matrix and adhesion molecules and identified 8 commonly dysregulated (3 downregulated and 5 upregulated) genes in CD133+ versus CD133− cells isolated from at least 2 of 3 HCC cell lines (Huh7, PLC8024, and SNU182 cells). This evidence concerns the gene PROM1 and hepatocellular carcinoma.