To uncover the specific methylation markers for RB, we utilized a public dataset GSE57362 containing 67 RBs, 12 normal retina, 8 non-proliferative diabetic retinopathy (DBT), 8 neuroretina, 9 Fibrovascular membranes from diabetic retinopathy (FVM) and 27 peripheral blood samples from patients with RB. This evidence concerns the gene ESCO2 and proliferative diabetic retinopathy.