As an extension of our previous studies and given the relevance of the CD4+CD45RBhi T cell transfer model of colitis to human disease, we examined the necessity of Foxp3, IL-10, and TGF-β in suppressing colitis in mice and show that ET required the presence of TGF-β and IL-10 but not Foxp3+ Tregs to alleviate colitis. The gene discussed is CD4; the disease is colitis.