We estimated the percentage and number of T lymphocytes (CD3+ CD19-), CD4+ T lymphocytes (CD3+ CD19- CD4+), CD8+ T lymphocytes (CD3+ CD19- CD8+), and the CD4+/CD8+ T lymphocyte ratio in two sepsis mouse models using flow cytometry (Figures 1D, E). The gene discussed is CD8A; the disease is Sepsis.