For example, elevated glucose decreased SND1, and leptin decreased RBM22, but insulin-like growth factor 1 (IGF-1) increased RAVER1, and palmitic acid increased PTBP1 and RBM22. Thus, this study provided evidence that not only could the overexpression of SRSF4, RBM45, SND1, and RAVER1 that is seen in the three molecular clusters enhance the development of NAFLD, but also that the metabolic milieu could contribute to the altered RNA splicing. Here, SND1 is linked to metabolic dysfunction-associated steatotic liver disease.