To investigate and understand the mechanisms relating to atherosclerosis, the zebrafish mutants (gucy1a1, gucy1a1, gucy1a1 + cct7, gucy1a1 + gucy1b1, gucy1b1 + cct7, and gucy1a1 + gucy1b1 + cct7) could be treated with high cholesterol diets to measure the effects on cholesterol levels, lipid deposits, and downstream effects of the PDEs. The gene discussed is CCT7; the disease is atherosclerosis.