Consistent with these results, the density PECAM-1-positive capillaries (Figures 1D,E) and α-SMA-positive arterioles (Figures 1F,G) in ischemic gastrocnemius muscles 14 days after induction of ischemia was significantly increased in cilengitide 50 μg/kg-treated mice compared with that in vehicle- and cilengitide 50 mg/kg-treated mice. This evidence concerns the gene ACTA1 and ischemia.