SLC12A2 and central nervous system cancer: NaDCA decreases NKCC1 expression in rat thymocytes.20 GB cell accumulates [Cl-]i to levels ∼10-fold higher than average.44 GB cell [Cl−]i homeostasis disruption is related to upregulated NKCC1.45 A potential NKCC1 role is a participation in cell proliferation in tumors with high NKCC1 expression, such as glioma.46 In human glioma, NKCC1 protein expression positively correlates with tumor grade.44 Pharmacological inhibition of NKCC1 reduces glioma cell migration and invasion.44,47 Differences in the effect of 10 mM and 5 mM NaDCA on U87 MG and PBT24 cells may be due to the force of Na+ ions.