RYR2 and colonic neoplasm: As shown in Figure 2A, the intersection genes with high mutations were APC, TP53, TNN, KRAS, MUC16, MUC4, SYNE1, FLG, FAT4, OBSCN, FAT3, RYR2, PIK3CA, FBXW7, DNAH11, MUC5B and ZFHX4. To further investigated whether these 17 commonly mutated genes were associated with TMB, colon cancer patients from TCGA cohort were classified into wild group and mutation group based on the 17 gene mutation status.