GSEA was performed, and the results showed that pathways were significantly enriched in the MUC4 mutant group, including antigen processing and presentation, cytosolic DNA sensing pathway, prion diseases, graft versus host disease, type I diabetes mellitus, leishmania infection, toll like receptor signaling pathway, natural killer cell mediated cytotoxicity and prostate cancer (Figure 5A). This evidence concerns the gene MUC4 and prostate cancer.