Loss-of-functional experiments were performed in HBV-expressing HCC cells in vitro, and circ-RNF13 expression was specifically silenced in Huh7-HBV and Hep3B-HBV cells after exogenous transfection of si-circ-RNF13 than si-NC (Figure 2A). This evidence concerns the gene RNF13 and hepatocellular carcinoma.