As for top 10 selected hub genes finally fit into TMB-IF, key genes were identified as FYB, CSF2RB, CTSS, CYBB, VCAM1 and SYK. Cathepsin S silencing (CTSS) has been proved to have a potential role of inducing apoptosis of HCC cells [36]. This evidence concerns the gene CYBB and hepatocellular carcinoma.