Western-blot assay for evaluation of proteins involved in drug resistance, EMT, apoptosis and PI3K/AKT pathway revealed that miR-95-3p improved GC progression by up-regulating MDR1 and ABCC1, stimulating the EMT process, increasing expression of Bcl2 and Bcl-xl and activating the PI3K/AKT pathway. The gene discussed is AKT1; the disease is gastric cancer.