CAMK2G and ischemia: To better investigate ischemia-induced oxidation of CaMKII under physiological conditions, the authors soaked acutely prepared mouse hippocampal slices in a modified artificial cerebrospinal fluid buffer pre-saturated with 95% N2/5% CO2 to mimic the ischemia condition, and a similar oxidative modification of CaMKII was observed in the hippocampal slices exposed to ischemic insults [71].