By comparing the characteristics of in vitro and in vivo nuclide uptake and the comprehensive analysis of MACC1 and SPON2 protein levels in colorectal cancer, we found that the higher the 18F-FLT uptake, the stronger the cell transfer ability, the higher the protein levels of MACC1 and SPON2, 18F-FLT The level of uptake was significantly correlated with the expression of MACC1 and SPON2. This evidence concerns the gene MACC1 and colorectal cancer.