Functionally important, in vivo inhibition of RIPK1 by Nec-1s completely prevented lethal listeriosis in OTUB1LPC-KO mice and both Nec-1s treatment and combined OTUB1 and MLKL deletion significantly reduced liver damage in Lm-infected OTUB1LPC-KO mice and cell death of Lm-infected and TNF-stimulated HepG2 cells, respectively. This evidence concerns the gene TNF and listeriosis.