NAFLD and associated liver damage in DUSP3-KO mice are evidenced by the increased levels of AST and ALT, of cholesterol and TG, by the increased expression level of fatty acid synthase (FASN), PPARγ and by increased inflammation as suggested by the increased levels of IL6, TNFα and CXCL1 (Supplementary Fig. S4, Supplementary Tables S3 and S4 and data not shown) as well as the infiltration of CD45+ cells in the damaged livers (data not shown). This evidence concerns the gene TNF and metabolic dysfunction-associated steatotic liver disease.