For example, while the first evidence showing an association between a loss of Brn-3b and metabolic dysfunction (hyperglycaemia and insulin resistance) has been identified using constitutive Brn-3b KO mutants in vitro studies have confirmed that Brn-3b, is itself, regulated by key metabolites such as glucose, insulin and fatty acids, which are dysregulated in obesity and T2D20. The gene discussed is POU4F2; the disease is Insulin resistance.