NKX6-1 and Hyperglycemia: To evaluate the effect of modest hyperglycemia on β-cell dedifferentiation with NKX6.1 inactivation, the non-diabetic samples were further divided into the non-prediabetic group with normal glycaemia (HbA1c < 5.7%) and the prediabetic group with modest hyperglycemia (HbA1c: 5.7–6.4%).